Developing dynamic substrates for studies of cell adhesion and migration

BIOL 37

Eun-ju Lee, Department of Chemistry, University of North Carolina at Chapel Hill, CB # 3290, Chapel Hill, NC 27599 and Muhammad N. Yousaf, mnyousaf@email.unc.edu, Department of Chemistry, University of North Carolina - Chapel Hill, CB #3290, Chapel Hill, NC 27599.
A class of model substrates has been developed for a study of cell adhesion and migration. These substrates can modulate the dynamic environment of biology and enable us to have spatial and temporal control of cell behavior. Our approach for the dynamic substrate is based on an electrically switchable self-assembled monolayer that presents redox active hydroquinone groups. In the presence of the cells, the surface can be activated to present biological ligand molecules by the chemoselective reaction between quinone form of the redox couple and soluble oxyamine-tethered ligands. We used these model substrates to study migratory behavior and internal structure of the cell by modulating cell population, pattern geometry, ligand density and ligand affinity.
 

Frontiers in Chemical Biology
5:00 PM-7:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biological Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007