Alternative synthesis, characterization, and DNA-binding of a [Ru(phen)2dppz]2+ phenazine linked dimer

INOR 174

Samantha Glazier, sglazier@stlawu.edu, Department of Chemistry, St. Lawrence University, Canton, NY 13617-1475 and Jennifer L. Achtyl, Department of Chemistry, St. Lawrence Universtiy, 23 Romoda Drive, Canton, NY 13617.
Dimer complexes show potential to be effective anticancer therapeutics because they exhibit large DNA binding constants and long residence times. Bengt Norden et al. previously reported extremely slow dissociation of a [Ru(phen)2dppz]2+ amide linked dimer from CT DNA, but detailed kinetics studies on residence times have not yet been reported. Here we report the synthesis and complete characterization (melting point, IR, and 1H-NMR) of the linker formed from the products of SeO2 reacted with 4-5-dimethy-o-phenylenediamine and 1,4-diaminobutane reacted with methylcyanoacetate. The resulting Se linker is then reacted with one or two [Ru(phen)2phendione]2+ complexes to form the monomer or dimer, respectively. Preliminary investigation of the dominant DNA-binding mode includes results from viscosity titrations, circular dichroism, and fluorescence spectroscopy.
 

Bioinorganic Chemistry: DNA and RNA
7:00 PM-10:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Inorganic Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007