CARB 73 |
| This study aims to prepare negatively charged electrospun fiber meshes for sustained release of alkaline chondrogenic growth factors. Both chondroitin sulfate (CS) and sulfated cellulose fiber meshes were prepared. To enhance CS-fiber mesh's aqueous stability, methacrylation of CS by glycidyl methacrylate (GMA) followed by photopolymerization was attempted. The crosslinked electrospun MA-CS fiber mesh, however, showed limited improvement in its aqueous stability. In parallel, regenerated cellulose fiber mesh obtained by the hydrolysis of electrospun cellulose acetate fiber mesh was oxidized by NaIO4, and subsequently coupled with sodium 2-aminoethylsulfate via reductive amination to introduce sulfate residues. The cellulose-derived, CS-mimicking electrospun fiber mesh was stable in water. SEM, FTIR and XPS analysis were performed to characterize the morphology and chemical composition of the fiber mesh. The sulfated fiber mesh pre-absorbed with chondrogenic growth factors is analyzed for its ability to induce chondrogenic differentiation of chondrocyte progenitor cells in culture. |
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General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Sci-Mix
Division of Carbohydrate Chemistry |