BIOL 179 |
| Drosophila melanogaster CTP: Phosphocholine cytidylyltransferase (CCT) catalyzes the conversion of phosphocholine and CTP to CDP-choline, a central reaction for the biosynthesis of phosphatidylcholine, a major component of the phospholipid bilayer. In higher eukaryotes, there are commonly two isoforms of CCT, denoted Dros1 and Dros2 in D. melanogaster. The Dros2 isoform has previously been characterized (Helmink and Friesen, BBA 1683, 78-88) and shown to be lipid-activated. A Drosophila melanogaster cDNA library was used for PCR amplification of the 1581 nt cDNA encoding Dros1. Using a baculovirus system, Dros1 CCT was expressed in Sf9 cells and purification of the 6x-His-tagged enzyme utilized metal affinity chromatography. Cation usage and lipid activation of Dros1 CCT were studied and kinetic parameters determined. Future studies will include determining the location of these isoforms as well as their role in the development of cells. |
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |