Increasing data quality and metabolite identifcation by integration of plate based radioactivity and automated nanoelectrospray mass spectrometry

ANYL 348

Gary A. Schultz, schultzg@advion.com1, Jonathan L. Josephs2, and Mary F. Grubb2. (1) Technology Development, Advion BioSystems, Inc, 22 Thornwood Drive, Ithaca, NY 14850, (2) Discovery Biotransformation, Bristol-Myers Squibb, Hopewell, NJ
The detection and quantitation of metabolites in a drug metabolism study is commonly by radioactivity detection after incubation or administration of radiolabeled compound. Radiodetection may be achieved by fraction collecting into 96 well plates, evaporating to dryness and reading with a radioactivity detector. The LC column effluent was split, with 80% going to a 4 96-well plate fraction collector at 15 s intervals and 20% going to a TriVersa NanoMate (Advion BioSystems) for online nanoelectrospray with simultaneous fraction collection in a 384-well plate on a Thermo LTQ mass spectrometer. Software was used to import and map the radioactivity data for the 96-well plates to the 384-well plate. Nanoelectrospray to generate MSn spectral trees was automatically performed for the components identified by radioactivity counting. The software provided a simple and robust method for converting the microscintillation plate data into a “chromatogram” which is correctly aligned with the “in-line” nanoelectrospray LC/MS data.
 

Analytical Technology for Drug Discovery
8:30 AM-11:50 AM, Tuesday, August 21, 2007 BCEC -- 105, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007