CMC determination of detergents by synchronous fluorescence and UV-Vis spectroscopy

ANYL 85

Daoyong Yu, ydyzwm@hdpu.edu.cn, Center for Bioengineering and Biotechnology, College of Chemistry & Chemical Engineering, China University of Petroleum, Qingdao, Shandong 266555, China and Shuguang Zhang, Shuguang@mit.edu, Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307.
There are a large number of methods applied to the determination of critical micelle concentration (CMC) of detergents, but usually each method only apply to some type of detergents and/or with some shortcomings. We propose a new sensitive method by synchronous fluorescence combined with UV-Vis absorption spectra, which don't need any additive and can be used to nearly all types of detergents in theory. The synchronous fluorescence spectra of detergents are different between in micelle and in monomer. Thus, plotting the fluorescence intensity at a particular wavelength against detergent concentration can obtain a slope change corresponding to the CMC, and the wavelength should be selected under considering both fluorescence and absorption spectra in order to obtain the best sensitivity. This novel method has been successfully applied to the CMC determination of a non ionic detergent Triton X-100, a lipid-like detergent Fos-Choline-14, and some peptide detergents (peptergents).
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007