Novel platforms for single cell microscopy

ANYL 441

Ute Neugebauer, ute.neugebauer@dcu.ie1, Tia E. Keyes, tia.keyes@dcu.ie2, Obianuju Inya-Agha2, Agnieszka Michota-Kaminska3, Niamh Moran4, Dermot Kenny4, Sarah O'Neill4, and Robert J Forster, robert.forster@dcu.ie2. (1) Dublin City University, Biomedical Diagnostic Institute, Glasnevin, Dublin 9, Ireland, (2) School of Chemical Sciences, National Centre for Sensor Research Dublin City University, Glasnevin, Dublin 9, Ireland, (3) Dublin City University, National Centre for Sensor Research Dublin City University, Glasnevin, Dublin 9, (4) Clinical Research Centre and Clinical Pharmacology, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland
Fast and reliable analytical detection methods are required to solve many complex biomedical problems. For example, thrombotic events are known to be associated with the activation of platelet integrins that involve both protein conformation change and changes in the surface density of the integrin. Here, we report on novel platforms for non-invasive imaging of whole cells including surface Enhanced Raman (SERS), surface Enhanced resonant Raman (SERRS) and luminescence. These platforms are based on functionalised metal nanoparticles that use the nanoparticle's properties, e.g., size, separation or surface plasmon, to enhance the spectroscopic signature of the proteins directly, or to enhance advanced dye labels based on transition metal complexes. Moreover, strategies for optimising the surface chemistry so as to maintain the cells, e.g., platelets, in an active state, are discussed. The application of these advanced platforms to platelet imaging before and after activation is reported.
 

Analytical Approaches
9:00 AM-12:00 PM, Thursday, August 23, 2007 BCEC -- 104A, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007