ANYL 110 |
| Inflammation is implicated in the pathogenesis of a number of diseases, including cardiovascular disease. Current research is focused on developing assays to search for biomarkers for inflammation. Eicosanoids are the oxidative metabolites of arachidonic acid (eicosatetraenoic acid), a long chain polyunsaturated fatty acid common in Western diets. AA can be oxidized by one of three pathways to form prostaglandins (PG), leukotrienes (LT), or a number of hydroxy and epoxy compounds. These eicosanoids have a variety of physiological functions, including regulating inflammation. We present a validated analytical method using LC-MS to separate and quantitate 23 different eicosanoids. This method uses negative electrospray ionization as the ion source and separation was performed on a 25 cm C18 column using a gradient elution of 0.1% formic acid and acetonitrile as the mobile phase. The method was applied to rat tissues to investigate the biochemical role of inflammation and its effects on cardiovascular health. |
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General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Analytical Chemistry |