Acrolein reacts with deoxyguanosine to form both the 8-hydroxy- (1) and 6-hydroxy-1,N²-propano (2) adducts.  However, only 1 has been unambiguously identified and quantified from acrolein treated DNA and intact cells.  We have developed a sensitive and selective UPLC/MS/MS method for the identification and quantification of both adducts.  Calf thymus DNA was incubated with acrolein over a range of concentrations for 24 h at 37^o C, then subjected to enzymatic digestion.  Adduct 2 co-eluted with dA, which obscured its identification. However, adduct 2 was clearly visible after the hydolysate was treated with adenosine deaminase.  The acrolein adducts were quantified by the stable isotope dilution method, and the level of 2 was determined to be ~25 % that of 1.