Effect of sequence context on rate and yield of interstrand crosslinks generated by abasic sites in duplex DNA

TOXI 66

Derrick R. Seiner, drs4zc@mizzou.edu1, Sanjay Dutta, sd967@mizzou.edu1, and Kent S. Gates, GatesK@missouri.edu2. (1) Department of Chemistry, University of Missouri-Columbia, 601 South College Avenue, Columbia, MO 65211, (2) Departments of Chemistry and Biochemistry, University of Missouri - Columbia, 601 S. College Ave., Columbia, MO 65211
Abasic sites are one of the most common lesions in cellular DNA. We recently characterized an interstrand crosslink generated by an abasic site in duplex DNA. In the work presented here, we have examined the effects of sequence context on the rate and yield at which a crosslink forms with a guanine residue in the 5'-CAp-3' sequence. In addition we report on the generation of abasic-site-derived interstrand crosslinks with nucleobases other than guanine.
 

Poster Session and Awards
6:00 PM-10:00 PM, Tuesday, August 21, 2007 BCEC -- 204 A/B, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Chemical Toxicology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007