Direct visual detection for a human papillomavirus DNA using bioconjugated silica nanoparticles

ANYL 119

Jingyu Piao, jypiao04@snu.ac.kr1, Eunhee Park, cute821224@hotmail.com1, Doo Soo Chung, dschung@snu.ac.kr1, Panyun Park, pypark@bio.bmelab.co.kr2, Jongwon Kim, jwkim@bmelab.com2, and Jihyoung Lee, jhl@bmelab.com2. (1) Department of Chemistry, Seoul National University, seoul, 151-747, South Korea, (2) Ste.811, Gyeonggi Technopark, 1271-11 Sa-dong Sangnok-gu, Gyeonggi-do 426-901, Ansan, 426-901, South Korea
A simple, sensitive DNA chip detection method based on the aggregation of plain silica nanoparticles is described. DNA of human papilloma virus (HPV), the major etiological agent of cervical cancer, served as a screening target in this study. Sandwich DNA hybridization reaction occurs between the capture oligonucleotide, the target HPV DNA, and the probe oligonucleotide conjugated with a silica nanoparticle, which renders spots on a DNA chip readily distinguishable to the naked eye. Scanning electron microscopy images of the spots clearly showed the aggregation of silica nanoparticle probes. Additional aggregation of the silica nanoparticle probes in a dendrimer-like format further improved the visibility of the corresponding spots. Direct visualization of 100 pM target DNA molecules was demonstrated. The described HPV DNA assay does not require any specialized optical detection instrumentation or highly trained technical personnel and will ease the development of point-of-care diagnostic devices.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

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8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007