Determination of the isoelectric points of neutral and basic amino acids by 13C NMR spectroscopic method

CHED 226

Yoo Mi Kim, jshin@qcc.cuny.edu and Jun H. Shin, jshin@qcc.cuny.edu. Department of Chemistry, Queensborough Community College, 222-05, 56th Avenue, S-445, Bayside, NY 11364
Since the 13C NMR spectroscopic method was introduced to determine the isoelectric point of amino acid, more amino acids (neutral and basic) have been studied for their isoelectric points. An Anasazi EFT 60 MHz FT-NMR spectrometer has been used to perform the project because deuterated solvent was not required for the instrument. Also the 13C NMR spectroscopic method was very useful to measure the pKa of carboxylic acids. The isoelectric points of amino acids determined by 13C NMR spectroscopic method were compared with the corresponding literature values.