Analysis of vitamin C in fruit, vegetables and juices via high pressure liquid chromatography

CHED 174

Yu Ha, sranganathan@qcc.cuny.edu, Syamala Ranganathan, sranganathan@qcc.cuny.edu, and Pedro Irigoyen, pirigoyen@qcc.cuny.edu. Department of Chemistry, Queensborough Community College, 222-05 56th Avenue, Bayside, NY 11364
The High Performance Column Chromatography (HPLC) procedure that measures quantitatively vitamin C in fruit, vegetables and commercial juices is described. The analytical protocol and effect of various buffers in the sample analysis is presented. Comparison of various types of vitamin-C containing food and beverages is made.