Structural and kinetics study on the interactions between Heparin/HS and dengue virus envelope protein

CARB 67

Fuming Zhang, zhangf2@rpi.edu1, Boyangzi Li, lib4@rpi.edu1, Zhenqing Zhang, zhangz5@rpi.edu1, Robert J Linhardt, linhar@rpi.edu1, and Rory Marks2. (1) Departments of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th Street, Troy, NY 12180, (2) Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109
Heparin and heparan sulfate have been shown to interact with a number of biologically important proteins thereby playing an essential role in the regulation of various physiological and pathological processes. The understanding of these interactions at the molecular level is important for the design of new highly specific therapeutic agents. Dengue virus is a human pathogen that has reemerged as an increasingly important public health threat. We found that the cellular receptor utilized by dengue envelope protein to bind to target cells is a highly sulfated type of heparan sulfate. In the present study, we report the kinetics study on the interactions between heparin/HS and dengue envelope protein using SPR and the structural analysis of heparin/HS binding to dengue envelope protein by LC-MS.
 

General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Carbohydrate Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007