Explosives detection by HPLC-based immunoextraction coupled with LC-MS and CE

AGRO 51

Raychelle Burks, rmburks@hotmail.com, Department of Chemistry, University of Nebraska-Lincoln, 705 Hamilton Hall, Lincoln, NE 68588-0304, Daniel D. Snow, dsnow1@unl.edu, Water Sciences Laboratory, University of Nebraska, 103 Natural Resources Hall, Lincoln, NE 68583-0844, and David S. Hage, dhage@unlserve.unl.edu, Department of Chemistry, University of Nebraska, 704 Hamilton Hall, Lincoln, NE 68588-0304.
The detection of explosives such as TNT and RDX has become an area of national priority due to the increased use of such substances in criminal activities and the rise in decontamination or remediation projects for closed military bases. The nature of these explosives and analyses requires measurement methods that can provide reliable identification of the explosives in complex sample matrices. Immunoextraction has long been recognized as a high selective approach for environmental samples. This research focuses on the development of HPLC-based immunoextraction columns for TNT and RDX that can be employed along with the methods of LC-MS or capillary electrophoresis. The immunoextraction columns contained anti-TNT or anti-RDX antibodies for the isolation of TNT, RDX, and their degradation products. Frontal analysis was used to characterize the binding properties of these columns. LC-MS analysis of these extracts was performed using an APCI ion trap. Analysis by capillary electrophoresis was performed using micellar electrokinetic chromatography (MEKC) using a mixture of sodium dodecyl sulfate and crowns as the pseudo-stationary phase.
 

New Developments and Issues in Agrochemical Sciences
8:00 AM-11:00 AM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Agrochemicals

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007