ANYL 44 |
| In the strive for utterly sensitive label-free affinity-based bioanalytical sensors, the LSPR concept is one of the most promising transducer principles for miniaturized array-based sensors of the future. Compared with discrete nanoscale features of noble metals, substrates composed of holes in thin noble metal films have the advantage of providing high sensitivity in the void of the hole. Using new means of selectively modifying either the bottom of the holes (composed of e.g. SiO2, TiO2 or ITO) or the planar metal substrate (e.g. Au, Ag or Pt) biorecognition reactions can be steered to predefined nanoscale regions.[1,2] Successful positioning of either planar supported lipid bilayer (SLB) patches or lipid vesicles to the holes will be presented, together with examples on using such and similar templates to probe lipid-membrane mediated biorecognition reactions, ranging from DNA hybridization to virus detection. Improvements in the instrumental resolution of low-cost LSPR sensors will be discussed,[3] and new means of using nanoplasmonics to determine the optical properties of lipid bilayers will be presented. References 1. Marie R, Dahlin AB, Tegenfeldt JO, Hook F: Biointerphases 2007, accepted. 2. Dahlin A, Zach M, Rindzevicius T, Kall M, Sutherland DS, Hook F: Journal Of The American Chemical Society 2005, 127:5043-5048. 3. Dahlin AB, Tegenfeldt JO, Hook F: Analytical Chemistry 2006, 78:4416-4423. |
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Surface Plasmon Resonance
1:30 PM-5:00 PM, Sunday, August 19, 2007 BCEC -- 105, Oral
Division of Analytical Chemistry |