Smart array of polymer beads using a removable polymer template on the glass surface

POLY 414

JongHwan Lee1, Okgene Kim1, JaeYoen Jung1, Kyunga Na1, Duckjong Kim2, Sangjin Park2, and Jinho Hyun, jhyun@snu.ac.kr1. (1) Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, San 56-1 Sillim-Dong, Gwanak-Gu, Seoul, South Korea, (2) Micro/Bio Fluidics Team, Korea Institute of Machinery & Materials, Daejeon, South Korea
In the presentation, we describe a simple method to fabricate an array of micro-scale beads conjugated with nanobiomolecules onto a glass surface using a removable polymer layer. Micropatterns of a removable polymer were transferred onto the spun-cast adhesive thin layer cured with UV irradiation by microcontact printing. The adhesion performance was controlled by UV doses and optimized at 40~140 mJ/cm2 for the stable microstructure of the beads. Smart polymer beads functionalized with nanobiomolecules were obtained by covalently binding stimuli-responsive biopolymer on the surface and used for the smart immunoassay of prostate specific antigen. Bead-based microarray has been widely used in molecular diagnostics, proteomics, and genomics to analyze multiplex biological materials. In recent, the bead-based microarray system is requested to be simple in fabrication and multifunctional in detecting and quantifying biological samples. This new method enables the simple fabrication of bead-based microarray and responds to the external stimuli by adopting nanobiomolecules showing its reversibility
 

Synthetic and Biological Macromolecules for Emerging Nanotechnologies
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Polymer Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007