BIOL 95 |
| Recently, there has been an increased interest in single nucleotide polymorphisms (SNPs) due to the biological and clinical importance. Fluorescence-labeled DNA probes have widely used in SNPs typing methods. 1, 8-Diaminonaphthyridine (DANP) derivative, whose emission spectrum was shifted by 30 nm in the presence of cytosine-bulged oligonucleotide, was used for the detection of pyrimidine-bulged oligonucleotide. We here report the synthesis and fluorescence properties of DANP-conjugated DNA probes for SNPs detection. DANP derivatives having aminoalkyl groups were synthesized as described previously and incorporated via carbamate linkage into 5'-hydroxy group of oligodeoxynucleotides attached on solid support (DANP-ODNs). DANP-ODNs selectively emit a strong fluorescence in the presence of fully matched duplex, which has cytosine opposite DANP. These results indicated that DANP-ODNs were useful probe for the detection of cytosine-related SNPs. |
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |