CARB 58 |
| In order to enhance the efficiency of antisense molecules for target RNA regulation, novel photo-reactive antisense oligonucleotides were developed. Previously, we reported photo-reactive oligonucleotides containing an adenosine whose 2'-OH was modified with 4,5',8-trimethylpsoralen (psoralen) via a methylene linker (2'-Ps-met) cross-linked with the complementary RNA (match-RNA) and scarcely did with the RNA having a single mismatch base(mismatch-RNA). In order to examine the relationship between tether identity and photo-cross-linking efficiency, we designed photo-reactive oligonucleotides containing 2'-O-psoralen-conjugated adenosine derivatives with various linkers such as an amidomethylene linker (2'-Ps-amd), an ethoxymethylene one (2'-Ps-eom), a propoxymethylene one (2'-Ps-eom) and a butoxymethylene one (2'-Ps-bom). We evaluated the photo-cross-linking efficiency of the photo-reactive oligonucleotides with match-RNA. The efficiency of photo-cross-linking and the sequence selectivity largely depended on the structure and the length of the linker. |
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General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Carbohydrate Chemistry |