Fourier transform coherent anti-Stokes Raman scattering microscopy

ANYL 341

Jennifer P Ogilvie, jogilvie@umich.edu, Meng Cui, mcui@umich.edu, and Joshua Skodack. Department of Physics/Biophysics Research Division, University of Michigan, 450 Church St, Ann Arbor, MI 48109
Coherent anti-Stokes Raman scattering (CARS) microscopy has experienced a recent renewal of interest due to its promise of providing detailed chemical information based on endogenous contrast. We present an interferometric time-domain Fourier transform implementation of coherent anti-Stokes Raman scattering (FTCARS). The method gives high resolution CARS spectra over the large bandwidth of a single femtosecond laser source (1500 cm-1), providing the ability to image multiple vibrational modes simultaneously. FTCARS employs a collinear geometry, and allows for straight-forward removal of the non-resonant background. We discuss recent progress towards improving the sensitivity of FTCARS microscopy for biological applications.