Recent progress in single-biomolecule fluorescence imaging

PHYS 1

W. E. Moerner, wmoerner@stanford.edu, Department of Chemistry, Stanford University, Stanford, CA 94305-5080
Optical imaging and analysis of single molecules continues to unfold as a powerful way to study the individual behavior of biological systems, unobscured by ensemble averaging. Selected recent progress in the following areas will be described: new single-molecule fluorophores for cellular imaging, self-quenched intermolecular dimer detectors of target DNA, single-molecule imaging in cells with superresolution position localization, and single-molecule studies of chaperonin function on surfaces and in solution using the ABEL trap. This work was performed in collaboration with the J. Frydman, R. J. Twieg, P. Wender, and L. Shapiro labs, and the author acknowledges the tireless efforts of J. Biteen, A. E. Cohen, N. Conley, J. Cui, W. Duim, M. Koenig, A. Kurtz, L. Lau, H.-L. Lee, S. Y. Lee, S. J. Lord, and N. Sinha.