Development of a sample preparation method for the metabonomic analysis of volume-limited plasma samples from selenium exposed fish

ANYL 114

Fang Fang, ffang001@student.ucr.edu1, Xin Deng, dengx@ucr.edu2, Silas SO. Hung, sshung@ucdavis.edu3, Daniel Schlenk, daniel.schlenk@ucr.edu2, and Cynthia K Larive, clarive@ucr.edu4. (1) Department of Chemistry, University of California, Riverside, CA 92507, (2) Department of Environmental Sciences, University of California-Riverside, Riverside, CA 92507, (3) Department of Animal Science, University of California-Davis, Davis, CA 95616, (4) Department of Chemistry, University of California-Riverside, PSI 323, Riverside, CA 92521
Selenium contamination of the San Francisco Bay-Delta Estuary has been suspected to cause a decline in the population of the Sacramento splittail fish. Metabonomic analysis of splittail fish plasma can help reveal new information about the effects of selenium exposure and improve our understanding of the biochemistry of selenium toxicity. To apply integrated 1H NMR and UPLC-MS analysis of these volume limited (70~150 ěL) fish plasma samples, a sample preparation method has been developed for effective deproteination, desalting and suppression of matrix effects. Our sample preparation method was developed using three types of plasma (human, rat, bovine) treated with four sample preparation procedures [centrifugal ultrafiltration, protein precipitation, liquid-liquid extraction, solid phase extraction (SPE)] and analyzed by 1H NMR followed by LC-MS. Our results indicated that acetonitrile protein precipitation provided both sample clean up and concentration for metabonomic analysis, while SPE was necessary for suppression of matrix effects for analysis by LC-MS.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007