Design and synthesis of fluorescent probes for detecting unlabeled nucleic acid and nonaqueous environment

ANYL 89

Liming Huang, huangl@unr.nevada.edu and Suk-Wah Tam-Chang, tchang@unr.edu. Department of Chemistry, University of Nevada, Reno, NV 89557
In this paper, we describe the design, synthesis, and study of perylenemonoimides as molecular probes that exhibit absorption and fluorescence properties that are dependent on the solvent and microenvironment. Furthermore, the fluorescence emission of these compounds in aqueous buffer solution is substantially increased in the presence of double-stranded DNA. Fluorophores that show different fluorescence properties in different pH and solvent environments are potentially useful for probing the microenvironment of proteins and for detection of unlabeled nucleic acids.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007