MEDI 341 |
| Radiation is one of the major therapies in cancer treatment. The main obstacle in treating cancer by radiation is cancer resistance. Therefore, developing novel approaches to reverse cancer resistance or to increase cancer sensitivity to radiation is an ongoing research effort. In this poster, we would like to report that an increase in oxidative stress by inhibition of glutathione reductase (GR) significantly increased the sensitivity of OVCAR-3 cells, a human ovarian cancer cell line, to radiation. When the cells were treated with 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonyl-amino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA), an irreversible GR inhibitor developed in this laboratory, followed by radiation in a 96 well plate, a significant synergistic effect was observed suggesting that inhibition of GR could be a novel approach to increase ovarian cancer sensitivity to radiation. The synergistic effect was correlated with an increase in cell oxidative stress which was measured by the levels of intracellular reduced glutathione and oxidized glutathione. |
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Poster Session
7:00 PM-9:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Medicinal Chemistry |