Nonlinear optical imaging of melanocytes in collagen matrix

ANYL 199

Kai Zhang, kaiz@berkeley.edu1, Wenkai Zhang, wkzhang@berkeley.edu1, Chia-Yi Yang, chiayi@ntumc.org2, and Haw Yang, hawyang@berkeley.edu1. (1) Department of Chemistry, University of California at Berkeley, Berkeley, CA 94720, (2) Department of Dermatology, Taipei City Hospital, Taipei, Taiwan
Nonlinear optical microscopy can achieve three-dimensional high-resolution images of tissue components with high sensitivity and specificity. We combine two-photon excited fluorescence (TPEF), four-wave mixing (FWM), and optically active sum-frequency generation (OA-SFG) for simultaneous imaging of unstained skin tissues. Intrinsic fluorophores in cancerous cells contribute to high-contrast TPEF images, whereas collagen fibers with large nonlinear susceptibility generate sizeable FWM signals. Our studies reveal different formation patterns in two clinically distinct skin tissue biopsies and show possible influence of collagen fibers by the growth of cancerous cells. Our result demonstrated that the simultaneous nonlinear imaging through TPEF, FWM and OA-SFG serves as a promising new approach for early diagnosis of skin cancer.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007