ANYL 248 |
| The development of ultrasensitive detection of DNA has attracted considerable attention for the applications of detection of disease markers and infectious agents, for diagnosis and treatment of diseases and for bioterrorism. Carbon nanotube based electronic DNA sensors have shown great promise in identifying DNA targets. Here we report a new DNA sensing scheme by taking advantage of the unique near infrared absorption feature of single-walled carbon nanotubes. A sandwich DNA hybridization essay is used to load streptavidin functionalized SWNT tags on to streptavidin coated magnetic beads. Target DNA serves as a linker molecule to attach the SWNTs to the magnetic beads via two biotinylated DNA probes. We are able to observe the SWNT near infrared signals at very low target DNA concentrations. In combination with other optical methods such as surface-enhanced Raman and nondegenerate four wave mixing spectroscopy, SWNTs may have great promise for optical DNA sensing applications. |
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General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Sci-Mix
Division of Analytical Chemistry |