Synchrotron radiation based nuclear resonant scattering (NRS) of enzymes and model compounds containing iron and nickel

INOR 939

Yisong Guo, ysguo@ucdavis.edu1, Yuming Xiao, yxiao@ucdavis.edu1, Hongxin Wang, hongxin@popper.lbl.gov1, Yoshitaka Yoda2, Xiaowei Zhang3, and Stephen P. Cramer, spcramer@ucdavis.edu4. (1) Department of Applied Science, University of California-Davis, Engineer III 3001, One Shields Ave., Davis, CA 95616, (2) Japan Synchrotron Radiation Research Institute, Japan, (3) Photon Factory, KEK, Japan, (4) Department of Applied Science, UC Davis / Lawrence Berkeley National Laboratory, MS 6-2100, 1 Cyclotron Rd., Berkeley, CA 94720
Nuclear resonant scattering of synchrotron radiation has achieved great success over the last two decades in studying hyperfine interactions and vibrations in solids. Recently, it has also been demonstrated as a powerful tool for studying minerals in earth's mantle through high-pressure diamond anvil cells. In this work, the first attempt at using 61-Ni synchrotron radiation perturbed angular correlation spectroscopy (SRPACS) and 57-Fe nuclear forward scattering spectroscopy (NFS, or synchrotron Mössbauer) on Fe-S proteins and inorganic model compounds have been presented. In 61-Ni SRPACS spectra, nuclear decay signals of Ni foil and Ni ferrite were observed up to 80 ns. In 57-Fe NFS spectra, 1 microsecond synchrotron bunch mode has been utilized, and the nuclear decay signal of ferrocene was observed up to 800 ns. The results showed that these techniques have great potential in studying metal sites, Fe and Ni, in two biologically important enzymes, nitrogenases and hydrogenases.

 

Spectroscopy of Inorganic Systems
9:00 AM-11:20 AM, Thursday, August 23, 2007 BCEC -- 211, Oral

Division of Inorganic Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007