NanG5 encoding an O-glycosyltransferase, is responsible for attached the 4-O-methyl-L-rhodinose on the hydroxy group of polyether

BIOL 226

Tiangang Liu, Tiangang_Liu@Brown.edu, Department of Molecular Biology and Department of Chemistry, Shanghai Jiaotong University and Brown University, Box H, 324 Brook Street, providence, RI 02912, David E. Cane, david_cane@brown.edu, Department of Chemistry, Brown University, 324 Brook Street, Providence, RI 02912, and Zixin Deng, zxdeng@sjtu.edu.cn, Laboratory of Microbial Metabolism and School of Life Science& Biotechnology, Shanghai Jiaotong University, Huashan Road 1954, Science building 3rd Floor, Shanghai, 200030, China.
Nanchangmycin is one kind of polyether ionophores, produced by Streptomyces nanchangensis NS3226. It is comprised by the polyether skeleton included thirty carbon atoms and a sugar moiety of 4-O-methyl -L-rhodinose. This sugar is very important because it give nanchangmycin more toxic to the parasite and less toxic to the host than the other polyethers. There are three glycosyltransferases, nanG5, nanG6 and nanG7 have been found in the nanchangmycin biosynthsis gene cluster. At first, the three genes' transcription have been checked by RT-PCR, and proved that nanG5 is constitutive gene. And then, the three glycosyltransferases were inactivated by PCR-Targeting respectively. As the result, NanG5 is responsible for attached the sugar moiety on the hydroxyl group of the glycoside, and the successful experiment of complementation of single gene of nanG5 prove it adequately. And the novel polyether, desugar-nanchangmycin has been detected and confirmed by the LC-MS/MS.
 

Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biological Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007