Tissue engineered cartilage formation with 3-D and dynamic environments

POLY 505

Soo Hyun Kim, soohkim@kist.re.kr1, Youngmee Jung, winnie97@kist.re.kr1, Sang-Heon Kim, skimbrc@kist.re.kr1, Young Ha Kim, yhakim@gist.ac.kr2, and Byoung Goo Min, bgmin@snu.ac.kr3. (1) Biomaterials Research Center, Korea Institute of Science and Technology, P.O.Box 131, Cheongryang, Seoul, 130-650, South Korea, (2) Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, 1 Oryong, Buk, Gwangju, 500-712, South Korea, (3) Department of Biomedical Engineering, Seoul National University, 28 Yeongeon, Jongno, Seoul, 110-750
In our previous studies, it was confirmed that mechanical stimuli enhanced the development of engineered cartilage tissues in elastic mechano-active PLCL scaffolds. It is known that the three-dimensional spatial organization of cells and extracellular matrix is crucial to functional cartilage formation. The purpose of this study is to form substantial tissue engineered cartilage with hybridization of hydrogels and PLCL scaffolds in dynamic environment by mechanical stimulation. The BMSCs were seeded onto scaffolds suspended in a solution of hydrogels and the continuous compressive deformation was applied. From the results, the proper periodic application of dynamic compression and 3-dimensional environments of the hybrid scaffolds of fibrin gels and elastic PLCL scaffolds can encourage BMSCs to differentiate to chondrocytes, maintain their phenotypes and enhance GAGs production and consequently, improve the quality of cartilaginous tissue formed in vitro and in vivo. Figure 1. SEM images of cell-polymer constructs (S: scaffold, G: fibrin gels, C: cells)

 

Scaffolds and Matrices for Tissue Engineering and Regenerative Medicine Applications
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Polymer Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007