ANYL 382 |
| At the cellular level, a very few of protein molecules can induce a significant cellular response. At such low concentration (far below the disassociation constant), conventional tools are unable to effectively detect these crucial molecules, hindering the better understanding of vital roles of trace amount of protein molecules on single living cells and their related biochemical pathways. We have developed and characterized single nanoparticle sensors, and used single nanoparticle sensors to image and detect individual protein molecules on single living cells, to map its distribution density and coverage on single living cells, and to monitor its binding reaction in real time. Characterization of binding affinity of single nanoparticle sensor shows that antibody conjugated nanoparticles preserve the primary biological activity of antibody. The dynamic range of single nanoparticle sensors is 0-30 molecules per living cell. The detailed experimental design and prospective applications will also be discussed in this presentation. |
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New Chemical and Biosensing Approaches for Cellular Analysis
8:30 AM-12:15 PM, Wednesday, August 22, 2007 BCEC -- 104C, Oral
Division of Analytical Chemistry |