Elicitor-like effects of ultrasonic irradiation on key enzymes of Saccharomyces cerevisiae

BIOL 203

Lizhen Zhou, gzzhoulizhen@163.com, Bing Li, lcbingli@scut.edu.cn, Lin Li, felinli@scut.edu.cn, Ximei Zhang, and Xiaoxi Li, xxlee@scut.edu.cn. College of Light Industry and Food Science, South China University of Technology, 381#Wushan Road, Tianhe District, Guangzhou, 510640, China
Ultrasonic irradiation as disinfection technology especially in water treatment has been brought into study for years. The disinfection effect of ultrasound irradiation is mainly due to its ultrasonic cavitation, which should induce violent shock waves and also the formation of reactive oxidative species. Besides the destruction of cell structure which could make cells inactivated, the metabolism of cells must also be influenced by those effects induced by cavitation. Saccharomyces cerevisiae was chosen as the object. In this study, the effects of ultrasound irradiation on activities of some key enzymes in cell anti-oxidation system and enzymes in cell metabolism system were investigated. The cross-membrane fluxes of Ca2+ and K+ induced by ultrasound irradiation were also determined. The results showed that in general low doses ultrasound irradiation would improve the activity of enzymes, but large doses ultrasound irradiation would inactivate the enzymes. The natural cross-membrane ion fluxes were also changed by ultrasound irradiation, and it should in turn induce some changes in cell metabolism properties. Financial supports from NSFC(No.20436020), GDNSF(No.04105934) and GDNSF(phD program, No.05300175) are acknowledged.
 

Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biological Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007