CARB 62 |
| A series of 5-(1H-1,2,3-triazol-4-yl)-2'-deoxyuridine derivatives (X, Y, Z) has been synthesized and incorporated into oligonucleotides. These modifications show increasing thermal stability towards complementary DNA and RNA strands upon multiple incorporations of up to +5.1 °C per. modification relative to the unmodified duplexes. Decreases in thermal stability is observed with single incorporation of the monomers with up to −5.1 °C per. modification relative to the unmodified duplex. Molecular modelling indicates that the high difference in thermal stability is due to extended π-π stacking offered by the triazole moieties (monomer X) as well as the added aromatic moieties in case of monomers Y and Z. The monomers X, Y and Z were evaluated for their biophysical properties in terms of thermal stability, circular dichroism (duplex type) and pKa-values.
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General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Carbohydrate Chemistry |
