Extraction of the chitosan-glucan complex and chitosan from fungal cell wall and their application in tissue engineering

CARB 118

Nitar Nwe, nitarnwe@yahoo.com, Japan Society for the Promotion of Science (JSPS), Japan, Faculty of Chemistry, Materials and Bioengineering, and HRC, Kansai University, Suita, Osaka, 564-8680, Japan, Willem F. Stevens, wfstevens@gmail.com, Faculty of Science, Centex Shrimp Biotechnology, Mahidol University, Bangkok, 10400, Thailand, and Hiroshi Tamura, tamura@ipcku.kansai-u.ac.jp, Faculty of Chemistry, Materials and Bioengineering, and HRC, Kansai University, Suita, Osaka, 564-8680, Japan.
Gongronella butleri USDB 0201 was grown using solid substrate fermentation for 7 days. The biomass was collected and the chitosan/glucan complex was isolated by extraction with 11 M NaOH. Chitosan was obtained by digestion of this complex by alpha –amylase. The yield of the chitosan/glucan complex and chitosan were 25-30 and 8-10 g/100 g of mycelia respectively. Chitosan/glucan complex and chitosan were dissolved in 0.35 M acetic acid at 95oC and 30oC respectively and used for the preparation of scaffolds for cell culture by the freeze-drying method. Swiss mouse embryo fibroblast NIH/3T3 cells were grown on these scaffolds at 37oC in 5% CO2 environmental incubator. After 7 days the viable cells were stained green by fluorescein diacetate. It was observed that fibroblast cells were able to adhere, spread and proliferate normally and had their well known polygonal morphology on both scaffolds. Therefore the chitosan/glucan complex and chitosan obtained from fungal source are promising candidates as scaffold materials for tissue engineering.
 

Carbohydrate Chemistry and Biochemistry
8:30 AM-11:50 AM, Thursday, August 23, 2007 BCEC -- 162B, Oral

Division of Carbohydrate Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007