AGRO 49 |
| The spinosyns belong to a new family of insect control agents that are derived from a naturally occurring soil bacterium Saccharopolyspora spinosa. Spinosad, which comprises spinosyns A and D, is widely used on a variety of crops to control several orders of insects. Spinetoram, which contains derivatives of both spinosyns J and L, is being developed at Dow AgroSciences and is expected to be introduced to the market soon. A rapid and sensitive immunoassay for detecting all active ingredients of spinosad and spinetoram was developed. Rabbits were immunized with 5,6-dihydro spinosyn A, 3'-O-glutarate-thyroglobulin conjugate. All sera were screened against numerous HRP conjugated antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average I50 for spinosyn A was 0.09 ppb with a detection limit of 0.02 ppb. The resulting assay equally detects all four active ingredients of both spinosyn and spinetoram. No or minimal cross reactivity was found to other spinosyn metabolites. The assay was validated for industrial waste water monitoring. Results of validation and application in waste water matrix will be discussed. |
|
New Developments and Issues in Agrochemical Sciences
8:00 AM-11:00 AM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Poster
Sci-Mix
Division of Agrochemicals |