BIOL 170 |
| Room-temperature ionic liquids (RTILs) are organic salts consisting of positively charged cations and negatively charged anions with a melting point below 100 °C, which have been extensively evaluated as environmental-friendly solvents to replace conventional volatile organic solvents. A key feature of ILs is that most of its properties such as polarity, hydrophobicity and solvent miscibility can be modified through appropriate tailing of the cation and anion. By this way, it provides new opportunities for many biocatalytic processes which are impossible in traditional solvents.Concurrent researches on ionic liquids have already confirmed that they support a diverse range of reactions. But until now there are few reports about whole-cell biocatalysis in ILs. This work presents the use of imidazolium Ionic Liquids in biphasic system for the asymmetric reduction of 2'-chloro-acetophenone to (S)- 1-(2'-chloro-phenyl) ethanol catalyzed by yeast. The results suggest that an increase in alkyl group chain substituted on the cation ring corresponds to an increase in toxicity to yeast cell. It is also found that the ratio of ILs in the biphasic system influences the performance of the reaction distinctly. Under the optimal conditions, the maximum yield and e.e. value of product were 85.9% and more than 99.9% respectively. *Supported by Chinese National Natural Science Foundation (no.20506022) and Key Project of Chinese National Programs for Fundamental Research and Development (no.2003CB716008) |
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |