Liquid chromatography-mass spectrometry methods for clinical analytes

ANYL 303

Karen W. Phinney, karen.phinney@nist.gov1, Mary Bedner, mary.bedner@nist.gov2, Nathan G. Dodder, nathan.dodder@nist.gov2, Bryant C. Nelson2, Catherine A. Rimmer, catherine.rimmer@nist.gov2, Lane C. Sander, lane.sander@nist.gov2, Katherine E. Sharpless, katherine.sharpless@nist.gov2, Susan S-C Tai, susan.tai@nist.gov2, and Stephen A. Wise2. (1) National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, (2) Analytical Chemistry Division, National Institute of Standards and Technology, Mailstop 8392, Gaithersburg, MD 20899-8392
Clinical diagnostics based upon immunoassays are a valuable aid in diagnosing and monitoring disease. Reference materials and reference methods are generally accepted as the best approach for standardization of immunoassays. NIST has been actively developing reference methods and Standard Reference Materials (SRMs) to improve the reliability of routine clinical measurements. Previously, many reference methods were based upon gas chromatography-mass spectrometry (GC-MS). Liquid chromatography-mass spectrometry (LC-MS and LC-MS/MS) is now increasingly being used for these analytes. NIST has completed development of reference methods based on LC-MS/MS for estradiol-17â, cortisol, progesterone, testosterone, thyroxine (T4), and triiodothyronine (T3). More recently, methods have been developed for analytes of nutritional interest in serum, including carotenoids, vitamin D, folate, and B vitamins. These methods are being utilized for value assignment of serum-based SRMs. Many of these new methods incorporate stable isotope labeled internal standards for quantitation.
 

Chromatographic Separations in Clinical Chemistry
9:00 AM-11:50 AM, Monday, August 20, 2007 BCEC -- 104A, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007