ANYL 322 |
| Nanoparticle enhanced bio-sensing has received significant recent attention. We are evaluating the use of protein-labeled gold nanoparticle reagents for surface plasmon resonance (SPR) imaging in an array format. Previous work has attributed enhancements in nanoparticle assisted SPR to electronic coupling, increased surface roughness, and a greater refractive index of the binding moiety. We are exploring the role of increased avidity of the modified nanoparticles through multivalent interactions between the nanoparticle reagent and proteins on the microarray surface. Antibody modified gold nanoparticles are employed in various immunoassay formats. Multivalency is manifested in antibody capture experiments using nanoparticles of various sizes. Larger particles interact with immobilized antigens through a larger number of antibodies and yield overall tighter binding as shown in the figure. The detection limit for antigens is also monitored directly and indirectly using a sandwich assay.
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Biomarker Discovery
1:30 PM-4:20 PM, Monday, August 20, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |
