Stacking of nonnatural bases in DNA duplexes

CARB 59

Stephen A. Woski, swoski@bama.ua.edu, Cuiling Liu, and Dominic F. Qualley, quall001@bama.ua.edu. Department of Chemistry, The University of Alabama, Box 870336, Tuscaloosa, AL 35487
In order to examine base-base stacking interactions within nucleic acids, 15-mer oligodeoxynucleotides were prepared bearing a single 4-substituted phenyl or 5-substituted indole residue. Upon hybridization with complementary DNA, the stabilities of the resulting duplexes were probed with UV thermal denaturation experiments. Substituent effects on the hybridization free energies will be presented. Correlations between the structures of the modified residues and their stacking with flanking base pairs elucidate important relationships which are conserved regardless of size or substitutent orientation.
 

General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix

Division of Carbohydrate Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007