BIOT 472 |
| The development of protein purification processes has become a major bottleneck in the production of biopharmaceuticals. In order to tackle this situation robotic techniques for the screening of parameters which are relevant for the design of downstream processes, which could significantly reduce development time, have recently been developed. In this theme we developed strategies and miniaturized systems on a TECAN Evo Freedom platform covering the evaluation important process variables as well as the major unit operations in downstream processing. Next to batch operations such as solubility screens, ATPS, isothermal and kinetic finite bath data, we extended the systems capability to column screens operated under constant flow conditions. It is thus possible to use scale-down models of chromatographic columns as well as membrane stacks under controlled fluid flow conditions. A newly developed fraction collection device further enables us to take automatically fractions ranging from drop size to several hundred micro liters. In its current form 8 systems are operated in parallel. In this paper we introduce the technique, hardware and its application on HTS platforms. Finally, parameter estimation procedures for model based process development as well as a comparison to typical lab scale systems are addressed. The techniques are presented using industrial model systems. |
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Downstream Processing: High Throughput Screening in Process Development
2:00 PM-4:20 PM, Thursday, August 23, 2007 BCEC -- 107B, Oral
Division of Biochemical Technology |