BIOL 230 |
| Atomic force microscope(AFM) with good signal-to-noise, high spatial and time resolution, and flexible detection environments is an excellent equipment on biological sample in physiological condition with the lateral resolution about 0.5nm and the vertical one about 0.1nm. Results: Firstly we want to introduce the applications of AFM to the surface structure of molecular motor i.e. mitochondrial ATP synthase from the hearts of Bovine and Swine, and following with the application in the details of the protein molecules. Secondly,stress on the studies in physical properties of single protein molecular such as surface viscidity and flexibility, surface charge and magnetic domain distribution, intermolecular interaction and then share the results or the experiences from the work on proteins function and manipulation with some typical example for example the two dimensional crystal of F0 moiety which are constituted into the lipid double layers with KSV-Minitrough and statistic analysis from the classic physical theories. At last analysis the dialectic relationship between physical techniques and the improvement of science in history. Discussion: Although we have gotten the beautiful topographies and results, however, we reach the conclusion that it is necessary to improve and manage the possible instruments effectively and sufficient for the personalities, and given out the optimum research routes. In addition, we get the proper experimental condition through the multi-parameter detection in which encountered the kinds of beautiful but evil experimental results or phenomena which might have puzzled some scientists in this field and discover some gloss phenomena in the AFM topography. |
|
Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |