Molecular breeding of medicinal plant using single chain Fv gene

AGRO 2

Yukihiro Shoyama, shoyama@niu.ac.jp1, Waraporn Putalun, waraporn@kku.ac.th2, and Hiroyuki Tanaka, htanaka@phar.kyushu-u.ac.jp1. (1) Faculty of Pharmaceutical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan, (2) Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, 40002, Thailand
The gene of scFv of anti-solamargine monoclonal antibody was cloned and transfer to the expression plasmid. The expressed scFv protein was confirmed to have the affinity agains solasodine glycoside ELISA. The gene was induced to Solanum khasianum through Agrobacterium resulting hairly roots. After subculturing of hairly roots for one month on the solid medium, a green spot appeared from the hairly root cultures, then regenerated plantlet. Expression of scFv protein and the concentration of solasodine glycosides were determined by ELISA using anti-His tag monoclonal antibody and anti-solamargine monoclonal antibody, respectively. We discovered that transformation with the scFv fragment improves the concentration of solasodine glycoside resulting in at least 3 times increase compared to the control plant. This system established here may open up new plant breeding methodology.
 

Immunochemistry
8:40 AM-12:05 PM, Sunday, August 19, 2007 BCEC -- 259A, Oral

Division of Agrochemicals

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007