ANYL 117 |
| Novel molecular probes based on the cyanopyranyl moiety or phenol derivative were designed and synthesized for the detection of proteins. These probes did not produce any fluorescence emission in the absence of protein. On the other hand, strong fluorescence emission was observed after addition of BSA in all the probes, and color change of the solution containing phloroglucinol derivative was observed, too. These fluorescence spectral changes and color changes upon binding proteins were caused by the ICT process and solvatochromic effect. The calibration graphs indicated good straight lines, and the detection limit was found to be 100 ng/mL under the given assay conditions. To demonstrate the application of probes, proteins after separation using SDS-PAGE were stained by these reagents using simplified staining protocol, and the imaging of protein spots on gel was successful. The experimental results clearly showed that these probes are good protein indicators for high-throughput analysis of proteins. |
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General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Analytical Chemistry |