ANYL 329 |
| While the basic separation mechanisms of chromatography and electrophoresis are different, there are still some interesting parallels between them. Pressure is a primary factor controlling separation efficiency (theoretical plates) in liquid chromatography, while electrical potential is a primary factor controlling separation efficiency in electrophoresis. It is possible to increase the separation power of liquid chromatography through the use of smaller particles of packing material and the application of higher pressures for pumping mobile phase. In an analogous manner it is possible to increase the separating power of capillary electrophoresis through the application of higher electrical potentials. Alternatively, enhanced separations can be achieved by creating an "endless" separation system. In capillary electrophoresis, this can be accomplished by doing separations in a circular system (Cyclic Capillary Electrophoresis) or in a linear system with a counterflow (Flow Counterbalanced Capillary Electrophoresis). Similar improvements can be attained by doing liquid chromatography in a cyclic system (Recycling Chromatography). |
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ACS Award in Analytical Chemistry in Honor of James W. Jorgenson
8:30 AM-12:00 PM, Tuesday, August 21, 2007 BCEC -- 104C, Oral
Division of Analytical Chemistry |