Searching for the infamous trans-fat

CHED 230

Minh H. Tran, tran_m@denison.edu, Thomas A. Evans, evans@denison.edu, and Jordan L. Fantini, fantinij@denison.edu. Department of Chemistry and Biochemistry, Denison University, Ebaugh Laboratories, Granville, OH 43023
Our analysis of fatty acid methyl esters (FAME's) in introductory organic chemistry laboratory has focused on three sources of fats and oils: lard, soybean oil and partially hydrogenated soybean oil. FAME's from these three sources are analyzed by isothermal capillary GC. Students assign individual peaks in the chromatograms using web-based characterizations of the fatty acid constituents of lard and soybean oil. Equations for the hydrogenation reactions are developed. Evidence of the isomerization of cis- to trans-fatty acid is complicated by the difficulty of their chromatographic separation. However, the current nutritional attention to the presence of trans-fats in foods has created special interest in the results.