Development of cITP-NMR to facilitate structure elucidation of heparin-derived oligosaccharides

ANYL 115

Stacie L. Eldridge1, Albert K. Korir1, and Cynthia K Larive, clarive@ucr.edu2. (1) Department of Chemistry, University of California, Riverside, Riverside, CA 92521, (2) Department of Chemistry, University of California-Riverside, PSI 323, Riverside, CA 92521
Heparin oligosaccharides are highly sulfated linear polysaccharides that display a wide range of biological activities through interaction with proteins, including growth factors and chemokines. Elucidation of the relationships between biological function and the microstructure of these biopolymers remains a challenge, mainly due to their structural heterogeneity and the lack of sufficiently sensitive and specific analytical techniques. Microcoil NMR technology is a relatively inexpensive method of enhancing the mass sensitivity of NMR and allows characterization of mass-limited compounds. Coupling capillary isotachophoresis (cITP) to NMR detection improves the concentration sensitivity of microcoil probes, allowing the separation and identification of heparin oligosaccharides requiring only micrograms of material isolated from enzymatic digestion. In this study, a cITP-NMR spectral database of heparin disaccharides is developed to expedite identification of rare substructures. Electrospray-ionization mass spectrometry and capillary electrophoresis are used to provide complementary structural data. This approach will facilitate discovery of novel oligosaccharides of biological importance.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007