New detectors for separation methods

ANYL 356

Gary M. Hieftje, Francisco J. Andrade, Gerardo Gamez, Steven J. Ray, Jacob T. Shelley, and Joshua S. Wiley. Department of Chemistry, Indiana University, Bloomington, IN 47405
Especially with the current emphasis on “–omics”, the power of separations methods has become critical. In turn, this power depends partly on the availability or development of detection methods that can provide selectivity, sensitivity, and compatibility with current and newly introduced separations methods. In this presentation, two new methods for detection will be introduced and evaluated. The first is employed with two-dimensional gel separations and is capable of detecting the presence of proteins at femtomolar levels, of determining the amount or concentration of each protein, of detecting the presence of heteroatoms or metals in each protein, and of quantifying the metal/protein ratio. The second method employs a novel flowing afterglow source that can be used in conjunction with gas chromatography, liquid chromatography, or thin-layer chromatography, and is compatible with either mass or ion-mobility spectrometers.