Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

CARB 60

Andreas S. Madsen, asm@ifk.sdu.dk1, Dorthe Lindegaard1, Irina V. Astakhova2, Andrei D. Malakhov2, B. Ravindra Babu1, Vladimir A. Korshun2, and Jesper Wengel, jwe@ifk.sdu.dk1. (1) Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, 5000, Denmark, (2) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, 117997, Russia
Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2'-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.

 

General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Carbohydrate Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007