Preparation of surface reactive core-shell type nano-associates entrapping enzyme in the core

POLY 167

Akifumi Kawamura, akifumi@chem.osakafu-u.ac.jp1, Chie Kojima1, Michihiro Iijima2, Atsushi Harada, harada@chem.osakafu-u.ac.jp1, and Kenji Kono1. (1) Department of Applied Chemistry, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan, (2) Oyama National College of Technology, 771 Nakakuki, Oyama, Tochigi, 323-0806, Japan
Core-shell type macromolecular assemblies have received considerable attentions from both of fundamental and applied fields. We have investigated the design of core-shell type nano-associates formed from bioactive compounds and synthetic polymers. In this study, the reactive groups were introduced at the periphery of core-shell type nano-associates entrapping enzyme in the core. The graft copolymer, which was composed of poly(ethylene glycol) having acetal groups at the end as a graft chain and cationic poly(allyl amine) as a main chain was synthesized (PEG-g-PAA), and the obtained PEG-g-PAA was complexed with glucose oxidase. And then, the cross-linking was introduced in the core with glutaraldehyde and NaBH3CN. Nano-associates had average diameter of 30nm, and glucose oxidases entrapped in the nano-associates maintained its enzymatic activity without a significant loss. Further, the acetal groups at periphery of nano-associates were converted to aldehyde groups. Such surface reactive nano-associates might have potential utilities as novel functional materials.
 

General Papers: Functional Materials
6:00 PM-8:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Polymer Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007