Isolation and electrochemical activity evaluation of electricity generated bacteria

FUEL 126

He Lee, yujief@hit.edu.cn, Xin Wang, Yanling Yu, Fei Gao, and Yujie Feng, yujief@hit.edu.cn. Dept Environmental Science & Engineering, Harbin Institute of Technology, No.202, Haihe Road, Nangang District, Harbin, 150090, China
Microbial full cells (MFCs) was designed as a two-chamber system with the bacteria enriched with the sediments of Songhua River in anode chamber and carbon paper with 10% Pt catalyst was chosen as cathode. PEM (Proton exchange membrane) was installed between the chambers. The anode solution was feed with sodium acetate of 800mg/L. The MFC was operated in batch cycles and then in the continuous flow. A maximum voltage of stable voltage of 450 mV, power density of 141.45 mW/m2 and CE of 27% was achieved, while COD removal kept as 90%. To characterize bacterial population community structure, SEM was employed to detect the microbial micrograph and meanwhile, the anodic microorganism were enriched and analyzed with DGGE. Six bands were sequenced with 16rRNA gene library. Available enrichment process was constructed and microbial species were screened by Hungate method. The electricity activities of the isolations were evaluated with electrochemical method and MFC tests.