AGFD 76 |
| The determination of the isotope values of constituents is gaining increasing importance, especially in view of the increased demand for the authenticity control and origin determination of the production of essential oils and food. In this regard, it was possible to discriminate citrus essential oils from different origins using isotope ratio analysis by high resolution gas chromatography-mass spectrometry (HRGC-MS). The method has been developed for the determination of origin for citrus essential oils on the basis of isotope peak and molecular peak of monoterpene hydrocarbons. In this study, various factors concerning the applicability of the analytical method were examined. HRGC-MS and gas chromatography-millimass spectrometry (GC-milliMS) were employed for the verification. The measurement by GC-milliMS could precisely detect the minute difference in molecular mass and their isotope mass of monoterpene hydrocarbons from different origins. With the coefficient of variation of isotope ratio values less than 1%, it was found that the repeatability, reproducibility, sampling and storage variation are suitable for the normalization of the method. In addition, internal standard introduction and other factors evolving the analysis condition were also evaluated in this study. |
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General Papers
8:00 AM-1:00 PM, Tuesday, August 21, 2007 BCEC -- 255, Oral
Division of Agricultural & Food Chemistry |